Method for Harvesting and Transplanting Cells

ABSTRACT

The dual syringe with percutaneous cannula is a medical device for harvesting and transferring cells for autologous transplantation without the requirement of a surgical setting or ambulatory care. The dual syringe is a closed sterile system that allows for the harvesting syringe to harvest the cells/tissue from a first location of a patient which is then centrifuged to aggregate the cells. The aggregated cell pellet is transferred to a transfer syringe. The cell pellet is then reinjected by the transfer syringe into the patient at a second location for therapeutic purpose. The percutaneous cannula permits insertion of a cannula, with the assistance of a needle, without the need of a surgical incision or trocar.

CROSS-REFERENCES TO RELATED APPLICATIONS

This application claims the benefit of U.S. application Ser. No.15/606,641 filed May 26, 2017, U.S. application Ser. No. 15/502,814filed Feb. 9, 2017, PCT Application Serial No. PCT/US2015/039833 filedJul. 9, 2015, U.S. Provisional Application Ser. No. 62/092,022 filedDec. 15, 2014, and U.S. Provisional Application Ser. No. 62/022,511filed Jul. 9, 2014, which are all incorporated by reference herein.

STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

Not applicable.

1. FIELD OF THE INVENTION

The present invention relates to the field of medical devices,specifically syringes and aspirating cannulas.

2. BACKGROUND OF THE RELATED ART

Autologous stem cell transplant generally occurs in a highly sterileenvironment such as available in an operating room or ambulatorysurgical center. One of the common methods to obtain stem cells isthrough the use of aspirating cannulas. However, cannulas require asecond instrument to access the interior of the body for collection ofthe stem cells. For example, extraction of bone marrow stem cellsgenerally requires the insertion of a trocar and the cannula after thetrocar is in place. Similarly, extraction of stem cells from adiposetissues generally requires a scalpel incision into the patient beforeinsertion of the aspirating cannula. Moreover, once the desired cellsand tissues are harvested by the cannula, the cells or tissues are thentransferred to syringes or test tubes. These transfers significantlyincrease the opportunity for sterile breaks through introduction ofmanual transfers. An invention and method is desired to simply extractand collect desired cells from a patient in a sterile closed system andthen reintroduce the concentrated cells for therapeutic purpose.

The present invention simplifies the process for autologous stem celltransplant by utilization of a dual syringe with percutaneous aspiratingcannula that safely harvests cells and tissues without the need for asurgical procedure and by minimizing the opportunity for a sterilebreak. The percutaneous aspirating cannula utilizes a needle and atapered edge cannula wherein the needle and tapered edge allow for thepercutaneous aspirating cannula to be inserted directly into a patientwithout the need of surgical incision or insertion of a trocar. The dualsyringe is a closed system that allows for the harvesting syringe toharvest the cells/tissue which is then centrifuged to aggregate thecells wherein the isolated cell pellet is then transferred to a secondsyringe where it can then be reinjected to the patient for a desiredtherapeutic purpose.

BRIEF SUMMARY OF THE DRAWINGS

FIG. 1 is a side profile of the harvesting syringe.

FIG. 2 is a front profile of the harvesting syringe.

FIG. 3 is an isometric view showing the bottom of the dual syringe.

FIG. 4 is an isometric view showing the bottom of the dual syringe withthe transfer covering removed.

FIG. 5 is a cross section of the side profile of the harvesting syringe.

FIG. 6 is a close-up cross section of the side profile of the lower endof the harvesting syringe.

FIG. 7 is a profile view of the needle hub assembly.

FIG. 8 is a profile view of the cannula.

FIG. 9 is a profile view of the percutaneous cannula.

FIG. 10 is a close-up cross section of the side profile of the needlehub assembly.

FIG. 11 is an isometric view of the dual syringe with a standardhypodermic needle.

FIG. 12 is an isometric view of the dual syringe with the percutaneouscannula assembly.

FIG. 13 is an isometric view of the dual syringe with the cannula.

FIG. 14 is an isometric view of the transfer syringe with a standardhypodermic needle.

FIG. 15 is a flow chart of the method of autologous stem celltransplant.

DETAILED DESCRIPTION OF THE VARIOUS EMBODIMENTS

As seen in FIGS. 1 through 6 and 12, the dual syringe 1 comprises aharvesting syringe 10 and a transfer syringe 100. The harvesting syringe10 comprises a barrel 20, spacer 70, and harvesting plunger 90. Thebarrel 20 comprises a partially cylindrical sidewall 21 and bottom wall30 that define an interior cavity 60. The side wall 21 is shaped so thatit forms a hemi-cylindrical groove 22 that extends from the top end 23of the harvesting syringe 10 to the bottom end 24. The preferredembodiment of the groove is hemi-cylindrical but other shapes such astriangular or rectangular are envisioned. On the opposing end of thebarrel 20 from the bottom wall 30 is an opening 40. Handles 25 extendperpendicularly from the cylindrical sidewall 21 and away from theopening 40 at the top end 23 of the barrel 20. The handles 25 arepositioned 90 degrees from the hemi-cylindrical groove 22.

The interior surface 26 is of the cylindrical sidewall 21 is generallysmooth and corresponds in shape to the exterior surface 27 whichincludes the hemi-cylindrical groove 22. A ridge 28 is located along theinterior surface 26 of the cylindrical sidewall 21 near the top end 23.The cylindrical sidewall 21 is generally translucent and containsidentifying markings 29 to indicate the volume of material within theinterior cavity 60.

Still referring to FIGS. 1 through 6 and 12, a tubular shaped spacer 70having a cylindrical sidewall 71, a bottom wall 72, and top wall 73 ispositioned within the hemi-cylindrical groove 22 such that the bottomwall 72 of the spacer 70 and bottom wall 30 of the harvesting syringe 10are aligned. The top wall 73 of the spacer 70 is positionedapproximately at the midpoint of the hemi-cylindrical groove 22 of theharvesting syringe 10. An interior channel 74 extends from the top wall73 to the bottom wall 72 of the spacer 70A top port 75 is positioned onthe top wall 73 which creates a fluid pathway with channel 74. A syringelocking mechanism 76 having a channel 78 through its center ispositioned on the top wall 73 above the top port 75. The syringe lockingmechanism 76 may be female luer taper connection. A bottom port 77 ispositioned on the bottom wall 72. A fluid pathway exists from the bottomport 76, through the channel 74, through the top port 75 and through thechannel 78 of the syringe locking mechanism 76. The spacer 70 may bebonded, glued, or welded onto the barrel 20. Alternatively, the spacer70 and barrel 21 may be integral.

The harvesting syringe 10 has an outflow port 31 positionedapproximately in the middle of the of the bottom wall 30 and an inflowport 32 positioned in a portion of the bottom wall 30 opposite from thespacer 70 and aligned with the bottom port 77 and the outflow port 31.Both the inflow port 31 and outflow port 31 create a fluid pathway intothe interior cavity 60 of the barrel 20. A needle attachment 34,positioned on the exterior surface 33 of the bottom wall 30, has achannel 41 through its center such that a fluid pathway exists with theinflow port 32. The needle attachment 34 may be a male luer taperconnection. On the exterior surface 33 of the bottom wall 30 a transferwall 35 is formed around the outflow port 31 and transfer port 77. Atransfer cover 36 is attached to the transfer wall 35 and covers theoutflow port 31 and transfer port 77. The transfer wall 35 and transfercover 36 create a pathway 38 between the outflow port 31 and transferport 77.

As seen in FIGS. 5 and 6, the interior surface 37 of the bottom wall 30is sloped such that the interior surface 37 is shaped to funnel contentsinto the outflow port 31. The exterior surface 33 of the bottom wall 30corresponds to the same slope of the interior surface 37. The pathway 38may be selectively closed by a valve 39.

The harvesting plunger 90 has a stopper or harvesting plunger head 91connected to one end of a shaft 92. The opposing end of the shaft 92 hasa handle 93 which is shaped to generally correspond with the interiorsurface 26 of the cylindrical sidewall 31, including thehemi-cylindrical groove 22. The harvesting plunger head 91 is shaped tocorrespond with the interior surface 26 of the cylindrical sidewall 31including the hemi-cylindrical groove 22. The shaft 92 is screwed intothe plunger head 91 and the friction created by the plunger head 91 andthe interior surface 26 generally holds the plunger head 91 in placewhich allows the shaft 92 to be screwed in or out. The harvestingplunger head 91 is made of a malleable material such as a hard rubber tocreate a seal between the interior cavity 60 of the barrel 20 and theatmospheric environment existing above the plunger head 91. The ridge 28on the interior surface 26 inhibits removal of the harvesting plungerhead 91 from the interior cavity 60 of the barrel 20.

As seen in FIG. 12, the transfer syringe 100 comprises a barrel 101 anda transfer plunger 110. The barrel 101 is translucent and containsidentifying markings 102 to indicate the volume of material within thebarrel 101. The transfer syringe barrel 101 comprises a cylindricalsidewall 103 having an open top end 104 and a closed bottom end 105. Ahandle 106 extends from the barrel 101 at its top end 104. A port 107 ispositioned in the bottom end 105 of the syringe barrel 101 to allowaccess into the interior cavity 108 of the barrel 101. A needleattachment 109 having a channel through its center is affixed toexterior surface of the bottom end 105. The needle attachment 109 may bea male luer taper connection. A fluid pathway exists between the channelof the needle attachment 109, port 107, and interior cavity 108.

As seen in FIGS. 9 and 12, the percutaneous cannula assembly 200 iscomposed of a needle hub 210, a needle 201, and a removable cannula 250.As seen in FIGS. 7 and 10, the hollow tubular needle 201 is comprised ofcylindrical sidewall 202 having a beveled point 203 on one end and anopening 204 on the opposing end. A cavity 205 is formed by thecylindrical sidewall 202 and extends from the beveled point 203 to theopening 204.

As seen in FIGS. 7, 9, 10, and 12, the needle hub 210 contains a syringeconnecting portion 211 and a cannula connecting portion 230. The syringeconnecting portion 211 consists of a cylindrical sidewall 212 having aconnecting outlet 213 on one end and a needle receiving outlet 214 onthe opposing end. A radial flange 217 is positioned around theconnecting outlet 213 on the exterior face 216 of the cylindricalsidewall 212. The radial flange 217 may be consistent with a female luertaper connection. A cavity 215 is formed by the cylindrical sidewall 212and extends from the connecting outlet 213 on one end and a needlereceiving outlet 214. The needle 201 is permanently affixed to at leasta portion of the interior face of the cylindrical sidewall 212. Twowings 218 extend radially away from the exterior face 216 of thecylindrical sidewall 212. The wings 218 permit a user to grip and rotatethe needle hub 210. The wings 218 are positioned on the exterior face216 of the cylindrical sidewall 212 at a sufficient distance away fromthe radial flange 217 to allow the radial flange 217 to connect to theneedle attachment 34 and be screwed or positioned into place. A fluidcommunication pathway exists from the connecting outlet 213 to thebeveled point 203 of the needle 201.

As seen in FIG. 8, the cannula connecting portion 230 consists of acylindrical sidewall 231 having a connecting end 232 on one end and acannula receiving end 233 on the opposing end. The diameter of thecylindrical sidewall 231 of the cannula connecting portion 230 isgreater than the cylindrical sidewall 212 of the syringe connectingportion 211. The connecting end 232 is attached to the exterior face 216of the cylindrical sidewall 212 of the syringe connecting portion 211 atthe approximate midpoint of the cylindrical sidewall 212 of the syringeconnecting portion 211. Threads 234 are positioned on the interior face234 of the cylindrical sidewall 231 of the cannula connecting portion230. Threads 234 may be consistent with a male luer lock taperconnection. Radial ridges 236 are positioned on the external face 237 ofthe cylindrical sidewall 231 of the cannula connecting portion 230. Theradial ridges 236 assist in gripping the needle hub 210.

The distance between the interior face 234 of the cylindrical sidewall231 of the cannula connecting portion 230 and the exterior face 216 ofthe cylindrical sidewall 212 of the syringe connecting portion 211 is ofsufficient distance that a cannula or other syringe may be removablyattached to the needle hub 210.

As seen in FIG. 8, the cannula 250 is comprised of a tubular shaft 251attached to a cannula hub 252. The cannula hub 252 is tubular in shapewith a generally cylindrical sidewall 253 with a cannula receivingportion 254 on one end and outlet 255 on the opposing end. A radialflange 256 is positioned around the outlet 255 of the cylindricalsidewall 253. The radial flange 256 may be consistent with a female luertaper connection. One end of the tubular shaft 251 is attached to thecannula hub 252 at the cannula receiving portion 254. The other end ofthe tubular shaft 251 is a tapered outlet 258. The cylindrical sidewall253 and cannula shaft 251 form a cavity 257 between the outlet 255 andthe tapered outlet 258. The cannula shaft 251 and the tapered outlet 258have an inner diameter large enough to allow the needle 201 to passthrough. Ports 259 extend longitudinally through the sidewall of thetubular shaft 251. Ports 259 may have smooth edges for minimal tissuecleavage or sharp skived edges for maximum tissue recovery depending onthe desired use and desired tissue and/or cells to harvest. Ports 259may be oriented on one side or may extend generally around thecircumference of the tubular shaft 251. Depth markings 260 arepositioned along the outer face of the cannula shaft 251 that indicatethe distance from the respective marking to the tapered outlet 258.

As seen in FIGS. 11 through 14, the transfer syringe 100 is attached tothe harvesting syringe 10 through attachment of the needle attachment109 to the syringe locking mechanism 76 of the spacer 70. The barrel 101of the transfer syringe 100 partially fits within the hemi-cylindricalgroove 22 of the harvesting syringe 10. The harvesting plunger head 91is shaped to not interfere with the transfer syringe 100 or to prohibitthe screwing in of the transfer syringe 100 onto the syringe lockingmechanism 76. A pathway is created between the interior cavity 60 of theharvesting syringe 10 and the interior cavity 108 of the transfersyringe 100 by the outflow port 31, pathway 38, bottom port 77, interiorcavity 74 of the spacer 70, top port 75, needle attachment 109, and port107. The pathway 38 may be selectively closed by a valve 39. Valve 39may designed as a pressure sensitive valve which seals with positivepressure from outflow port 31 but opens upon negative pressure from thebottom port 77.

As seen in FIG. 12, the needle hub 210 may attach to the dual syringe 1through mating of needle attachment 34 of the dual syringe 1 and ridge217 of the needle hub assembly 210. The cannula 250 may attach to theneedle hub 210 through mating of the cannula connecting portion 230 andradial flange 256 of the cannula 250. As seen in FIGS. 9 and 12, theneedle 201 extends out the tapered outlet 258 of the cannula 250 whenthe cannula 250 is attached to the needle hub 210. As seen in FIG. 13,the cannula 250 may also attach to the dual syringe 1 through mating ofneedle attachment 34 of the dual syringe 1 and radial flange 256 of thecannula 250. In the preferred embodiment, the attachments are luer locktaper connections although alternative embodiments may use a luer sliptaper connection.

Referring to FIG. 15, the broad process 300 of operation of the dualsyringe 1 comprises the following steps:

Anesthetize the patient generally or locally where cells are to beharvested. Anesthetization may occur through use of the dual syringe 1with a standard hypodermic needle 50 attached as seen in FIG. 11, orthrough the percutaneous adipose aspirating cannula 200 as seen in FIG.12. Anesthetizing agent is drawn into the harvesting syringe 10 bypulling on the harvesting plunger 90. The anesthetizing agent is drawnthrough the standard hypodermic needle 50 or percutaneous adiposeaspirating cannula 200. The anesthetic agent is then applied locally atthe site of cell harvesting.

The dual syringe 1, with the attached transfer syringe 100 (with thetransfer plunger 110 fully depressed) and the percutaneous cannulaassembly 200, is inserted into tissue or bone of a patient to harvestthe desired cells. The beveled point 203 of the needle 201 along withthe cannula 250 inserts percutaneously through patient's skin andadvanced into the patient until the appropriate depth as indicated bythe depth markings 260 on the cannula 250. The tapered outlet 255 of thecannula 250 assists insertion into the patient's skin to reduce anycatching or blunt trauma.

Once the desired depth is obtained, the cannula 250 is unmated from theneedle hub assembly 210 which allows for the removal of the dual syringe1 (with the needle hub assembly 210 still attached) from the cannula250. The needle hub assembly 210 is unmated from the harvesting syringe10. The harvesting syringe is then attached to the cannula 250 throughmating the needle attachment 34 of the dual syringe 1 and ridge 256 ofthe cannula 250. This establishes a fluid communication pathway from thetapered outlet 258 and ports 259, through the tubular shaft 251, throughthe cannula hub 252, through the outlet 255, through the inflow port 32,and into the barrel 20 of the harvesting syringe 10.

The cannula 250 may then be used as a standard aspirating cannula toharvest the desired cells. The user creates negative pressure withinbarrel 20 by pulling on the harvesting plunger 90 to draw in cells andtissues while simultaneously moving the cannula within the patient'sbody at the desired location. As the cannula 250 is moved back and forthwhile inside the patient, the ports 259 and tapered outlet 258 shear andcollect cells/tissue which are then drawn into the cannula 250 and areultimately drawn into the barrel 20.

Once the desired volume of cells and/or tissues are harvested, theharvesting syringe 10 (with the cannula 250 still attached) is removedfrom the patient.

The cannula 250 is removed 270 from the harvesting syringe 10. The shaft92 is unscrewed from the plunger head 91 and removed from the harvestingsyringe 10. A standard syringe port cap is attached to the needleattachment 34 to seal inflow port 32. The cap ensures sterility andprevents migration of the cell pellet into the inflow port 32.

The dual syringe 1 is placed in a centrifuge and spun at approximately500 to 2000 g forces for a period of 3 to 20 minutes. Centrifugationcauses the aspirated cells to separate from the aspirated fluid. As aresult of the funnel shape of the bottom wall 30, the cells, denser thanthe fluid, form a pellet on the bottom wall 30 near the outflow port 31.The valve 39 remains closed due to the positive pressure exerted fromthe outflow port 31 which prevents any of the contents of the barrelfrom migrating through pathway 38 and/or through the bottom port 77 ofthe spacer 70.

The dual syringe 1 is removed from the centrifuge. The transfer plunger110 is pulled away from the bottom end 105 which creates negativepressure within the interior cavity 108 of the transfer syringe 100.This negative pressure opens the valve 39 to allow cells of the pelletto move through the outflow port 31, through the pathway 38, through theinterior cavity 74 of the spacer 70, through the top port 75, throughthe port 107 of the transfer syringe and into the interior cavity 108 ofthe transfer syringe 100. To adjust for the varying hydraulic pressurewithin the interior cavity 60 of the harvesting syringe 10, ifnecessary, the harvesting plunger may be manually depressed, afterreattachment of the shaft 92, by the user in conjunction with user'spulling of the transfer plunger 110.

Once the cell pellet is fully transferred to the transfer syringe 100,the transfer syringe 100 is removed from the syringe locking mechanism76 and a standard hypodermic needle is attached to the transfer syringe100 at the needle attachment 109 as seen in FIG. 14. The gauge anddesign of the needle for the transfer syringe may depend on the type oftissue delivered, the tissue the needle needs to penetrate to deliverthe cells and the volume of the cells to be delivered. Saline and/orother chemicals may be added to reconstitute the cell pellet in thetransfer syringe 100.

The transfer syringe 100 is then inserted into a patient in a specificlocation for delivery of the harvested cells for therapeutic purposes.

A person of ordinary skill in the art would appreciate the number,gauge, and design of the ports 259 may vary depending on the type oftissue harvested, the tissue the needle needs to penetrate to acquirethe tissue, and the volume of the tissue needed.

The dual syringe may be used with a standard aspirating cannula as wellwith traditional access to the cells created through trocar use orsurgical incision. Once the cells are harvested, the dual syringe isremoved from the patient. The standard aspirating cannula is removed 270from the harvesting syringe 10. The shaft 92 is unscrewed from theplunger head 91 and removed from the harvesting syringe 10. A standardsyringe port cap is attached to the needle attachment 34 to seal inflowport 32. The dual syringe 1 is placed in a centrifuge and spun atapproximately 500 to 2000 g forces for a period of 3 to 20 minutes.Centrifugation causes the aspirated cells to separate from the aspiratedfluid. As a result of the funnel shape of the bottom wall 30, the cells,denser than the fluid, form a pellet on the bottom wall 30 near theoutflow port 31. The valve 39 remains closed due to the positivepressure exerted from the outflow port 31 which prevents any of thecontents of the barrel from migrating through pathway 38 and/or throughthe bottom port 77 of the spacer 70. The dual syringe 1 is removed fromthe centrifuge. The transfer plunger 110 is pulled away from the bottomend 105 which creates negative pressure within the interior cavity 108of the transfer syringe 100. This negative pressure opens the valve 39to allow cells of the pellet to move through the outflow port 31,through the pathway 38, through the interior cavity 74 of the spacer 70,through the top port 75, through the port 107 of the transfer syringeand into the interior cavity 108 of the transfer syringe 100. To adjustfor the varying hydraulic pressure within the interior cavity 60 of theharvesting syringe 10, if necessary, the harvesting plunger may bemanually depressed, after reattachment of the shaft 92, by the user inconjunction with user's pulling of the transfer plunger 110. Once thecell pellet is fully transferred to the transfer syringe 100, thetransfer syringe 100 is removed from the syringe locking mechanism 76and a standard hypodermic needle is attached to the transfer syringe 100at the needle attachment 109 as seen in FIG. 14. The gauge and design ofthe needle for the transfer syringe may depend on the type of tissuedelivered, the tissue the needle needs to penetrate to deliver the cellsand the volume of the cells to be delivered. Saline and/or otherchemicals may be added to reconstitute the cell pellet in the transfersyringe 100. The transfer syringe 100 is then inserted into a patient ina specific location for delivery of the harvested cells for therapeuticpurposes.

The above device may be used for autologous stem cell transplantation inan office setting as described in FIG. 15. A healthcare provider mayutilize local anesthetic at the specific harvesting site or generalanesthetic for the harvesting of stem cells. Stem cell harvesting mayoccur through adipose tissue in a patient's abdominal region, or throughadipose tissue in another adipose-rich region such as the inner thigh orthrough a patient's bone marrow. The healthcare provider utilizes thesterile dual syringe and attaches the percutaneous cannula 200. Thehealthcare provider inserts the dual syringe 1 with the percutaneouscannula 200 as described in FIG. 15 into the patient's abdomen andaspirates 50 to 60 milliliters of adipose tissue into the harvestingsyringe 10. Centrifugation separates a cellular pellet rich inmesenchymal stem cells from the stromal vascular fraction. Themesenchymal stem cells are then transferred to the transfer syringe 100as described in FIG. 15. The transfer syringe is removed from theharvesting syringe, a needle is attached as shown in FIG. 11, and thenthe mesenchymal stem cells are transplanted into the same patient fortreatment of osteoarthritis, for assistance in wound healing, or for oneor more of many other regenerative medicine uses. The procedure isaccomplished in the same office visit for the patient.

In a preferred embodiment the harvesting syringe 10, transfer syringe100, percutaneous cannula assembly 200, syringe port cap, and hypodermicneedle 50 are sterile and stored in a single use sterile packaging kit.The packaged unit is designed for singular use for a single patient fortransplantation of autologous tissue.

We claim:
 1. A method for harvesting and transplanting cells comprising:attaching a percutaneous cannula assembly to a dual syringe assembly;said dual syringe assembly comprises a harvesting syringe with a plungerand transferring syringe with a plunger fluidly connected in a sterileclosed system; said percutaneous cannula assembly comprises: a needlehub comprising a syringe attachment member, cannula attachment member,and a hollow needle extending from said cannula attachment member; and acannula having an opening on one end and an attachment member on theopposing end wherein said attachment member of said cannula is connectedto said cannula attachment member of said needle hub; and said needle ofsaid needle hub is positioned within said cannula and extends throughsaid opening of said cannula; attaching said syringe attachment memberof said needle hub to said harvesting syringe; inserting saidpercutaneous cannula into a patient at a first location; aspiratingcells into said harvesting syringe by pulling on said plunger of saidharvesting syringe until desired amount of cells are aspirated;centrifuging said dual syringe to aggregate a cell pellet; transferringsaid cell pellet into said transfer syringe in closed system by pullingon said plunger of said transfer syringe until desired amount of saidpellet is transferred; detaching said transfer syringe from saidharvesting syringe; and attaching a needle to said transfer syringe; andinjecting said cell pellet at a second location.
 2. The method forharvesting and transplanting cells of claim 1 wherein said firstlocation is adipose tissue.
 3. The method for harvesting andtransplanting cells of claim 1 wherein said dual syringe assemblyfurther comprises: a harvesting syringe having a sidewall and bottomwall that define a cavity having an open end opposite said bottom wall;a first port and a second port in said bottom wall each of said portscreating a fluid pathway with said cavity; wherein said plunger of saidharvesting syringe is inserted into said cavity of said harvestingsyringe through said open end; said transfer syringe having a sidewalland bottom wall that define a cavity having an open end opposite saidbottom wall; a port in said bottom wall of said transfer syringecreating a fluid pathway with said cavity of said transfer syringe; andwherein said plunger of said transfer syringe is inserted into saidcavity of said transfer syringe through said open end; wherein saidsecond port of said harvesting syringe is in fluid communication withsaid port of said transfer syringe.
 4. The method for harvesting andtransplanting cells of claim 1 or 3 further comprising the steps of:disconnecting said needle hub from said cannula while said cannularemains in said patient; removing said needle hub from said dualsyringe; and attaching said dual syringe to said cannula.
 5. The methodfor harvesting and transplanting cells of claim 4 further comprising thesteps of: removing said dual syringe with cannula from patient; removingsaid cannula from said dual syringe; and removing said shaft from saidplunger of said harvesting syringe.
 6. A method for harvesting andtransplanting cells comprising: attaching a percutaneous cannula to adual syringe assembly; said dual syringe assembly comprises a harvestingsyringe with a plunger and transferring syringe with a plunger fluidlyconnected in a sterile closed system; said percutaneous cannulacomprises: a cannula having an opening on one end and an attachmentmember on the opposing end wherein said attachment member of saidcannula is connected to said harvesting syringe; inserting saidpercutaneous cannula at a first location; aspirating cells intoharvesting syringe by pulling on said plunger of said harvesting syringeuntil desired amount of cells are aspirated; centrifuging said dualsyringe to aggregate a cell pellet; transferring said cell pellet intosaid transfer syringe in closed system by pulling on said plunger ofsaid transfer syringe until desired amount of said pellet istransferred; detaching said transfer syringe from said harvestingsyringe; attaching a needle to said transfer syringe; and injecting saidcell pellet at a second location.
 7. The method for harvesting andtransplanting cells of claim 6 wherein said dual syringe assemblyfurther comprises: a harvesting syringe having a sidewall and bottomwall that define a cavity having an open end opposite said bottom wall;a first port and a second port in said bottom wall each of said portscreating a fluid pathway with said cavity; wherein said plunger of saidharvesting syringe is inserted into said cavity of said harvestingsyringe through said open end; said transfer syringe having a sidewalland bottom wall that define a cavity having an open end opposite saidbottom wall; a port in said bottom wall of said transfer syringecreating a fluid pathway with said cavity of said transfer syringe; andwherein said plunger of said transfer syringe is inserted into saidcavity of said transfer syringe through said open end; wherein saidsecond port of said harvesting syringe is fluidly connected to said portof said transfer syringe.
 8. The method for harvesting and transplantingcells of claim 7 further comprising the steps of: disconnecting saidneedle hub from said cannula while said cannula remains in said patient;removing said needle hub from said dual syringe; and attaching said dualsyringe to said cannula.